Equipment and
composition to be used (total weight 3.0 kg):
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An INC-12V
BioTherm portable incubator produced by CryoLogic, Australia with dimensions of
270x210x260 mm. This incubator runs on a chargeable battery for
between 36 and 48 hours, and can also run on 12V DC or 220V AC. This
incubator radiates no rays (harmful or harmless) of any kind.
-
Inside the
incubator will be twelve (12) culture tubes containing a culture medium of 5 ml
each. Additional culture medium of a volume of 150 ml will be included in
the incubator. There will also be a 150 ml tube for used culture medium (waist).
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Sterile
embryos, stem cells and somatic cells of mice and sheep will be inside the
culture tubes in two tubes each. The environment in which these embryos
and cells are cultured has to be 100% sterile and free of all known
microorganisms, including bacteria and viruses. The embryos and cells
therefore present no risk to human health or environmental contamination in the
event of a highly unlikely spillage. The embryos and cells will be
collected under sterile surgical procedures to ensure an absolutely
contamination-free environment, as well as experimental material.
Five sterile
syringes and fifty sterile needles to change half of the culture medium in each
tube every second day.
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